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KMID : 0368420060490040326
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Journal of Plant Biology
2006 Volume.49 No. 4 p.326 ~ p.326
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Characterization of an apple anthocyanidin synthase gene in transgenic tobacco plants
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Kim Sung-Hyun
Lee Jae-Rin
Kim Seong-Ryong
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Abstract
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Anthocyanin is the major color pigment in plants. The apple anthocyanidin synthase gene(ANS) manifests fruit skin-preferential expression (Kim et al., 2003). To understand the regulatory mechanism for such expression, we isolated and analyzed an appleANS genomic clone. Sequence analysis of ca. 1.4-kb of theANS promoter region predicted several cis-elements for MYB, light responsive GT-1, and the ABA Responsive Element (ABRE). Transgenic tobacco plants carrying a chimeric fusion between theANS promoter and the β-glucuronidase gene(GUS) showed that GUS was expressed in the receptacles and immature seeds as well as in the floral buds, but not in the vegetative organs.
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KEYWORD
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anthocyanidin synthase gene, anthocyanin, apple, GUS, promoter
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